Research Article

Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus

Figure 7

(a) Effect of loop primers (LP) on sensitivity and incubation duration. M-molecular ladder, 1‒30 minutes without LP; 2‒30 minutes with LP; 3‒45 minutes without LP; 445 minutes with LP; 560 minutes without LP; and 660 minutes with LP. (b) Specificity of the colorimetric-RVFV-UDG-RT-LAMP assay. M-100 bp molecular ladder, 14 RVFV strains G66, 1983, 5335, and G64, 5-PPR, 6-Capripox, 7-NTC.
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