Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus

Table 2

Details of the RT-LAMP Glycoprotein gene primer set for rapid and real-time detection of RVFV.

Primer’s name and description	Position on the genome^a	Length of the oligonucleotide (bp)	Oligonucleotide sequence
F3	2584–2602	19	GGG​TGC​ATA​AAC​TCA​CTC​T
B3	2775–2794	20	CGA​GGA​ATT​TCT​GAG​AAT​GG
FIP (F1c + F2)	2667–2635 2615–2652	42	GCT​GAC​TGA​ACC​CCA​GTT​TGT​CTT​TGA​TGG​CTC​TGT​TTC​AAC
BIP (B1c + B2)	2696–2715	40	GGA​CGC​AGA​GGG​CAT​TTC​AGCAT​CAA​CAA​TTG​CAT​ACC​CT
	2753–2773
LF	2636–2656	21	GAT​GAT​GCT​CCC​AAG​TCT​ACT
LB	2731–2752	22	CTT​TCA​TTG​AGA​GCC​CAG​GCA​A
F2	2615–2652	21	CTT​TGA​TGG​CTC​TGT​TTC​AAC
B2	2753–2773	20	CAT​CAA​CAA​TTG​CAT​ACC​CT
F1c	2667–2635	21	GCT​GAC​TGA​ACC​CCA​GTT​TGT
B1c	2696–2715	20	GGA​CGC​AGA​GGG​CAT​TTC​AG

^aRVFV ZH-501 strain (genebank accession code: M11157.1).