The SARS-CoV-2 UTR’s Intrudes Host RBP’s and Modulates Cellular Splicing

Table 1

List of primers used in study.

Name	Purpose	Target	Figure	Primer	Sequence (5′-3′)
SARS-CoV-2 5′ UTR	Cloning, overexpression validation	5′UTR	1, 3	F	TAA​GCAGCT​AGCATT​AAA​GGT​TTA​TAC​CTT​CCC​A
				R	TAA​GCAAAG​CTTCTT​ACC​TTT​CGG​TCA​CAC​C
SARS-CoV-2 3′ UTR	Cloning, overexpression validation	3′UTR	1, 3	F	TAA​GCAGCT​AGCCAA​TCT​TTA​ATC​AGT​GTG​TAA​C
				R	AAG​CAAAG​CTTGTC​ATT​CTC​CTA​AGA​AGC​T
MORC3	Splicing assay	E: 4-5	2(a), 2(b)	F	GGA​GCA​TGT​TGT​TGT​TCC​AA
				R	ATG​ATC​CTC​GTC​CCC​TTC​TT
THOC2	Splicing assay	E: 34-35-36	2(a), 2(b)	F	GAG​TCC​AGG​CAT​GAT​AAA​GAA​AA
				R	CTG​TGC​TTG​TCC​GAG​GAC​TT
HNRNPLL1	Splicing assay	E:5-6	2(a), 2(b)	F	TGG​CAA​AGT​GCA​ACG​TAT​TG
				R	TTC​TTG​GGT​AGG​GTG​TTG​AAA
DDX54	Splicing assay	E:18-19	1	F	GGG​ACA​GTC​AGG​ACA​GGA​AG
				R	GCT​CCT​GCT​GAA​GCT​CCT​C
CRNKL1	Splicing assay	E: 6-7-8	2(a), 2(b)	F	TGG​AGG​AAA​TGT​TGG​GAA​AC
				R	TTC​TTC​AAA​GCG​GGC​ATA​CT
Beta actin	Normalization		2(a), 2(b)	F	AGA​AAA​TCT​GGC​ACC​ACA​CC
				R	CTC​CTT​AAT​GTC​ACG​CAC​GA