Measuring the Cytochrome Nitrite Reductase Activity—Practical Considerations on the Enzyme Assays
Figure 2
(a) Progression curves for ammonia (filled symbols) and nitrite (open symbols) for ccNiR reaction, in the presence of equivalent electron concentrations of dithionite reduced mediators at C. MV (■□); DQ (♦◊); PS (); AQS () and IC (X). Enzyme concentration was 0.7 nM for MV, DQ, and PS assays, 7 nM for AQS, and 14 nM for IC. (b) Nitrite to ammonia conversion percentages in the presence of each mediator.
); AQS (
) and IC (X
). Enzyme concentration was 0.7 nM for MV, DQ, and PS assays, 7 nM for AQS, and 14 nM for IC. (b) Nitrite to ammonia conversion percentages in the presence of each mediator.
