Measuring the Cytochrome Nitrite Reductase Activity—Practical Considerations on the Enzyme Assays
Figure 6
(a) Amperomograms of PG electrodes with immobilized ccNiR, in the presence of increasing amounts of nitrite, recorded at (a) −440 mV, (b) −350 mV, and (c) −255 mV. (b) Michaelis-Menten fittings to the calibration curves performed at −440 mV (■),−350 mV (♦), and −255 mV (). The assays were carried out at C with an electrode rotation speed of 600 rpm. The solid lines represent the Michaelis-Menten simulations of enzyme kinetics.
). The assays were carried out at C with an electrode rotation speed of 600 rpm. The solid lines represent the Michaelis-Menten simulations of enzyme kinetics.
