DNA Binding and Antitumor Activity of α-Diimineplatinum(II) and Palladium(II) Dithiocarbamate Complexes
Figure 11
Competition between [Pt(bpy)(Oct-dtc)]NO3 and the inset for [Pd(bpy)(Oct-dtc)]NO3 with ethidium bromide for the binding sites of DNA (Scatchard plot). In curve no. 1, Scatchard plot was obtained with calf thymus DNA alone. Its concentration was 60 μM. In curves nos. 2, 3, and 4, respectively, 33 μM, 71 μM, and 111 μM for Pt(II) complex and 12 μM, 35 μM, and 55 μM, for Pd(II) complex were added, corresponding to molar ratio [complex]/[DNA] of 0.55, 1.18, and 1.85 for Pt(II) complex and 0.2, 0.58, and 0.92 for Pd(II) complex. Solutions were in 20 mM NaCl and 20 mM Tris-HCl (pH 7.0). Experiments were done at room temperature.
