Role of Superoxide Anions in the Redox Changes Affecting  the Physiologically Occurring Cu(I)-Glutathione Complex

<table>Assessment of GSSG by the EDTA-modified NADPH/glutathione reductase assay: correlation between GSSG and Cu(II)-GSSG. NADPH (0.2 mM), glutathione reductase (2 U/mL), and EDTA (2 mM) were added to solutions containing increasing concentrations of the Cu(II)-GSSG complex (5–60 <i>μ</i>M). Results are expressed as <i>μ</i>M GSSG concentration. The symbols represent: solutions containing the Cu(II)-GSSG complex (5–60 <i>μ</i>M) assayed in the presence (■) or in the absence of EDTA (□).</table>

Bioinorganic Chemistry and Applications

fig4

Figure 4

Figure 4: Role of Superoxide Anions in the Redox Changes Affecting  the Physiologically Occurring Cu(I)-Glutathione Complex 