Galectin-1-Dependent Mitochondria Apoptosis Plays an Essential Role in the Potential Protein Targets of DBDCT-Induced Hepatotoxicity as Revealed by Quantitative Proteomic Analyses

Figure 6

Effects of DBDCT on ROS generation and NF-κB activation with different concentrations for 24 h and effects of NAC (inhibitor of ROS) and PDTC (inhibitor of NF-κB) on the expression of Gal-1 induced by DBDCT in HL7702 cells. (a) ROS generation. (b) NF-kB activation. (c) Effects of inhibitor on the expression of Gal-1 induced by DBDCT (2 μmol/L). (A) (D) Effects of NAC (1, 5, and 10 μmol/L) on Gal-1 protein and mRNA level. (B) (E) Effects of PDTC (5, 10, and 20 μmol/L) on Gal-1 protein and mRNA level. (C) (F) Effects of the combination of NAC (5 μmol/L) and PDTC (10 μmol/L) on Gal-1 protein and mRNA level. Results were normalized according to GAPDH level. Each value represents the mean ± SD of three independent experiments. , , and compared with the control. , , and compared with the DBDCT alone.