Galectin-1-Dependent Mitochondria Apoptosis Plays an Essential Role in the Potential Protein Targets of DBDCT-Induced Hepatotoxicity as Revealed by Quantitative Proteomic Analyses

Figure 8

Effects of DBDCT on HL7702 cells proliferation and apoptosis, and the analysis of caspase-3, -8, and -9 activities. (a) Morphological changes of HL7702 cells by microscopic observation under an inverted light microscope (magnification: 20×). (b) Apoptosis of HL7702 cells. (A) The percentage of apoptotic cells was detected by flow cytometry using annexin V/PI double staining. (B) Apoptosis was assessed by counting the percentage of annexin V-positive cells. (c) Proliferation of HL7702 cells. (d) Analysis of caspase-3, -8, and -9 activities. HL7702 cells were treated with DBDCT alone for 24 h or induced by OTX008 (10 μmol/L) for 3 h firstly to get a low Gal-1 level and then treated with DBDCT for 24 h. Each value represents the mean ± SD of three independent experiments. compared with the control group. , , and compared with the DBDCT alone.