Synthesis and Optimization of Deesterified Acacia-Alginate Nanohydrogel for Amethopterin Delivery

Table 2

Experimental process parameters and response summary of nanohydrogel preparation.

Exp. runs	DEA solution concentration (w/v %)	Alginate molecular weight (MW)	ATN/DEA ratio (w/w %)	Particle size (nm)	Zeta potential (mV)	S/N ratio of size	S/N ratio of zeta potential
1	0.008	3	1 : 4	148.50	20.5	43.4345	26.2351
2	0.008	6	1 : 8	180.64	22.3	45.1363	26.9661
3	0.008	9	1 : 12	250.70	24.6	47.9831	27.8187
4	0.008	12	1 : 16	195.28	27.2	45.8132	28.6914
5	0.016	3	1 : 8	300.12	26.8	49.5459	28.5627
6	0.016	6	1 : 4	354.17	27.9	50.9842	28.9121
7	0.016	9	1 : 16	211.34	23.6	46.4996	27.4582
8	0.016	12	1 : 12	408.97	28.4	52.2338	29.0664
9	0.024	3	1 : 12	412.07	28.9	52.2994	29.2180
10	0.024	6	1 : 16	325.78	26.7	50.2585	28.5302
11	0.024	9	1 : 4	187.34	22.4	45.4526	27.0050
12	0.024	12	1 : 8	267.58	24.9	48.5491	27.9240
13	0.032	3	1 : 16	439.04	29.5	52.8501	29.3964
14	0.032	6	1 : 12	425.18	28.6	52.5715	29.1273
15	0.032	9	1 : 8	378.19	25.2	51.5542	28.0280
16	0.032	12	1 : 4	312.67	26.4	49.9017	28.4321