Biosynthesis of Antitumoral and Bactericidal Sanguinarine

García, Víctor P.; Valdés, F.; Martín, R.; Luis, J. C.; Afonso, A. M.; Ayala, J. H.

doi:https://doi.org/10.1155/JBB/2006/63518

BioMed Research International

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Research Article | Open Access

Volume 2006 | Article ID 063518 | https://doi.org/10.1155/JBB/2006/63518

Biosynthesis of Antitumoral and Bactericidal Sanguinarine

Víctor P. García,^1,2F. Valdés,³R. Martín,³J. C. Luis,³A. M. Afonso,⁴and J. H. Ayala⁴

Received24 Jun 2005

Revised13 Oct 2005

Accepted04 Dec 2005

Published01 Mar 2006

Abstract

A simple, rapid, and reliable TLC method for the separation and determination of sanguinarine has been established. This intensively studied biologically active alkaloid has a wide range of potentially useful medicinal properties, such as antimicrobial, antiinflammatory, and antitumoral activities. Sanguinarine has also been incorporated into expectorant mixtures and has a strong bactericidal effect upon gram-positive bacteria, particularly Bacillus anthracis and staphylococci. These medicinal properties are due to the interaction of sanguinarine with DNA. A fibre-optic-based fluorescence instrument for in situ scanning was used for quantitative measurements. The sanguinarine was determined over the range 5–40 ng and a detection limit of 1.60 ng. The method was applied to the quantification of sanguinarine in tissue culture extracts of Chelidonium majus L.

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Copyright © 2006 Víctor P. García et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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