(a) Survival of stationary phase B. breve in 1% porcine bile. B. breve (closed circles) and B. breve (open circles). Overnight cultures were inoculated (3%) into GM17 and MRS broth containing 1% porcine bile. Viable cell counts were performed by serial dilution in one-quarter strength Ringer's solution followed by plating onto GM17Cm5 or RCMCm4, respectively. Standard deviations of triplicate results are represented by error bars. (b) Effect of bilE on the gastrointestinal persistence of B. breve (closed circles) and B. breve (open circles) were used for peroral inoculation of female BALB/c mice ( ). B. breve counts were determined in stools at 48-hour intervals, adapted from Watson et al. [20].
