Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor
Figure 5
Effect of GLP-1(7-36)amide on the initial rate of binding to the plasma membranes from CHO cells transiently expressing GLP-1 receptor and GFP-GLP-1 receptor. Mock (empty bar); GFP-GLP-1 receptor (filled bar); GLP-1 receptor (patterned bar). fmol/mg/min for CHO, 13.4 fmol/mg/min for GFP-GLP-1 receptor, and 9.8 fmol/mg/min for GLP-1 receptor (basal values, no GLP-1(7-36)amide added). Each bar with the indicated standard deviation represents the mean value of three independent experiments done in duplicate. ANOVA: F(5, 12) = 9.95; .001. Tukey-Kramer multiple comparison test was used to reveal significant differences between groups of interest. See Results for the details.
