Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor

<table>Effect of GLP-1(7-36)amide and forskolin on the production of cAMP from CHO cells transiently expressing GLP-1 receptor and GFP-GLP-1 receptor. GLP-1 receptor (empty bar); GFP-GLP-1 receptor (filled bar). <svg height="11.75" id="M127" style="vertical-align:-0.43678pt" version="1.1" viewbox="0 0 90.900002 11.75" width="90.900002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> fmol/mg  and 37.7 fmol/mg for GLP-1 receptor and GFP-GLP-1 receptor, respectively (basal value, no ligand added). Each bar with the indicated standard deviation represents the mean value of three independent experiments done in duplicate. ANOVA: F(5, 12)<svg height="11.175" id="M128" style="vertical-align:-0.20474pt" version="1.1" viewbox="0 0 57 11.175" width="57" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>.0001. Tukey-Kramer multiple comparison test was used to reveal significant differences between groups of interest. See Results for the details.</table>

BioMed Research International

fig6

Figure 6

Figure 6: Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor 