A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag

Table 1

Primers for whole gene synthesis of superfolder GFP.

No. and length	Oligo Sequences (5^′→3^′)
S1F 59	ATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGATTCTGGTTGAACTGGATGG
S2R 59	GCCTTCGCCGCGCACGCTAAACTTATGGCCATTAACATCGCCATCCAGTTCAACCAGAA
S3F 59	TAGCGTGCGCGGCGAAGGCGAAGGCGATGCGACCAACGGCAAACTGACCCTGAAGTTTA
S4R 59	AGGGTCGGCCACGGCACCGGCAGTTTGCCGGTGGTGCAAATAAACTTCAGGGTCAGTTT
S5F 59	CGGTGCCGTGGCCGACCCTGGTGACCACCCTGACCTATGGCGTGCAGTGCTTTAGCCGC
S6R 59	CGGACTTAAAGAAATCATGGCGTTTCATGTGATCCGGATAGCGGCTAAAGCACTGCACG
S7F 59	CATGATTTCTTTAAGTCCGCGATGCCGGAAGGCTATGTTCAGGAACGCACCATTAGCTT
S8R 59	TTTCACTTCCGCGCGGGTCTTATAGGTGCCATCATCTTTAAAGCTAATGGTGCGTTCCT
S9F 59	GACCCGCGCGGAAGTGAAATTTGAAGGCGATACCCTGGTGAACCGCATTGAACTGAAAG
S10R 59	TTATGGCCCAGAATGTTGCCATCTTCTTTGAAGTCAATGCCTTTCAGTTCAATGCGGTT
S11F 59	GCAACATTCTGGGCCATAAACTGGAGTACAATTTCAACAGCCATAACGTGTATATTACC
S12R 59	TTTTGAAATTCGCTTTGATGCCGTTCTTCTGTTTGTCCGCGGTAATATACACGTTATGG
S13F 59	ATCAAAGCGAATTTCAAAATCCGCCATAATGTGGAAGATGGCAGCGTGCAGCTGGCGGA
S14R 59	CACCGGGCCATCGCCGATCGGGGTATTCTGCTGATAGTGATCCGCCAGCTGCACGCTGC
S15F 59	GATCGGCGATGGCCCGGTGCTGCTGCCGGACAATCACTACCTGAGCACCCAGTCCGTGC
S16R 54	CATGTGATCGCGTTTCTCATTCGGATCTTTGCTCAGCACGGACTGGGTGCTCAG
S17F 54	TGAGAAACGCGATCACATGGTTCTGCTGGAGTTTGTGACCGCGGCGGGTATCAC
S18R 44	CTTGTACAGCTCGTCCATGCCATGAGTGATACCCGCCGCGGTCA