Research Article
A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag
Table 3
Purification results of sfGFP-TEV-His6 Nd1ā6 and TEV-His6 collected from 1-L of expression culture medium.
| Abbreviation | Theoretical MW (kDa) | Total protein after Ni-NTA (mg) | Purity | Total protein after High Q (mg) | Purity | Total activity (mol/h) | Specific activity (mol/h/mg) |
| Nd1 | 53.5 | | 90% | | 96% | | | Nd2 | 53.8 | | 94% | | 98% | | | Nd3 | 54.1 | | 93% | | 97% | | | Nd4 | 54.5 | | 93% | | 98% | | | Nd5 | 55.0 | | 93% | | 98% | | | Nd6 | 54.2 | | 93% | | 96% | | | TEV | 28.8 | | 98% | ā | ā | | |
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