BioMed Research International

Research Article

VHL Frameshift Mutation as Target of Nonsense-Mediated mRNA Decay in Drosophila melanogaster and Human HEK293 Cell Line

Table 1

Primer’s list used in this study.
NameSequence - Utilization
Upf1FTTGGAATCATCACGCCTTACGAUpf1 probe
Upf1RCATGCCAACCGGAACTGGCATGUpf1 probe
Upf1FmutATGTCTTGCGTGTGTTCTAACGAACGTMutagenesis
Upf1RmutACGTTCGTTAGAACACACGCAAGACATMutagenesis
Vh CCCTCAAGCCCTTCAGGAGGTGCGGGTGAACVh mutagenesis
Vh GGGAGTTCGGGAAGTCCTCCACGCCCACTTGVh mutagenesis
Vh GGATGCACGTACGCTGCAGAGGATCTTTCAVh mutagenesis
Vh TGAAAGATCCTCTGCAGCGTACGTGCATCCVh mutagenesis
Vh construct1(EcoRI)CGCGCGGAATTCATGCCCCGGAGGGCGGAGAACTConstruct1
Vh R construct1(XhoI)CGCGCGCTCGAGATGGTGAAACCCCGTCTCTACTConstruct1
Vh F1 construct1(XhoI)CGCGCGCTCGAGTCAGGGGAAATGGAGAAAATAGConstruct1
Vh R1 construct1(XbaI)CGCGCGTCTAGAGAGAATAGGATACAAAAAGATTGGAConstruct1
c.163delGFACTGGGCGCCGAGGAGGAGATGAGGCCGGGCGGCCGCConstruct1 mutagenesis
c.163delGRGCGGCCGCCCGGCCTCATCTCCTCCTCGGCGCCCAGTConstruct1 mutagenesis
Vh F construct2(EcoRI)CGCGCGGAATTCATGCCCCGGAGGGCGGAGAACTConstruct2
Vh R construct2(XhoI)CGCGCGCCTCGAGTCAATCTCCCATCCGTTGATGTGCConstruct2
c.172delGFCAGGTCATCTTCTGCAATCGCATCCGCGCGTCGTGCTConstruct2 mutagenesis
c.172delGRAGCACGACGCGCGGATGCGATTGCAGAAGATGACCTGConstruct2 mutagenesis
pVHLFGGCCGCCGCATCCAVHL QPCR
pVHL RCATCGTGTGTCCCTGCATCTCVHL QPCR
pGFP FGCAACTACAAGACCCGCGFP QPCR
pGFP RGTCGGCCATGATATAGACGGFP QPCR
rp49_FCACACCGGAAACTCAATGGATrp49 QPCR
rp49_RGGTCATCTTGAAGCTGGAAGGrp49 QPCR
Act4A_FGCTTCGCTGTCTACTTTCCAAct4A QPCR
Act4A_RCAGCCCGACTACTGCTTAGAAct4A QPCR
Adh_RT_FGGCGGTCCCGGTGGTART-PCR, Adh QPCR
Adh_RT_RCTGGTAGATGGCATTGAATCCRT-PCR, Adh QPCR