A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture
Figure 6
(a) Dividing the scaffold into thirds, the core of static cultured scaffolds was nearly void of cells by day 4. Cell density was preserved in the peripheral crust (superior and inferior thirds). (b) Scaffolds exposed to flow-perfusion culture exhibit a near homogenous cell density in all areas of the scaffold, with evident cell viability in the core, in contrast to the static cultured samples. (c) To account for the possibility of confounding shear stress or differences in cell proliferation in dynamic versus static culture, the core : periphery ratio was calculated.
