Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast
Figure 2
Specificity of the antibodies elicited against NUCKS’ peptide: Purified NUCKS ((a) lanes 2–4), HClO4-extracts invasive ductal carcinoma GII (Ca in (b): lanes 5 and 7) and MCF 7 cells ((b): lanes 6 and 8), and SDS-total lysates of normal breast (N: lanes 9, 10, 13, 14) and invasive ductal carcinoma GII (Ca in (c): lanes 11, 12, 15, 16) each matching the same patient (c) were separated by SDS-PAGE, blotted onto nitrocellulose and incubated with affinity purified anti-NUCKS antibodies. Lane 4: the antibodies were preincubated with the peptide used for antibody production and purification. Total protein was stained on blots with Amido Black (lanes 1, 2, 5, 6, and 9–12).
