Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a
Cre-Expression Vector
Figure 3
Cre-mediated excision in vivo.
(A): (a)–(c) Oviducts
transfected with pCE-29 alone. EGFP-derived green fluorescence (but not
HcRed1-derived red fluorescence) was observed. (d)–(f) Oviducts
transfected with pCRTEIL alone. HcRed1-derived red fluorescence (but not
EGFP-derived green fluorescence) was observed. (g)–(i) Oviducts
cotransfected with pCRTEIL and pCAG/NCre. Both types of fluorescence (indicated
by arrows in (h) and (i)) were seen, although green fluorescence appeared to be
weaker than red fluorescence. (j) and (k) Cryostat sections of oviducts sampled
1 day after introduction of pCRTEIL and pCAG/NCre DNA (0.2 g each) and subsequent in vivo electroporation. Note that
strong, but patchy, EGFP fluorescence presents in some oviductal epithelial cells (indicated
by arrows in (k)). (a), (d), (g), and (j) Photographs taken under light
(Light); (b), (e), (h), and (k): photographs taken under UV illumination using
filters for detection of green fluorescence (EGFP); (c), (f), and (i)
photographs taken under UV illumination using filters for detection of red
fluorescence (HcRed1). (B) Luciferase reporter gene activity in oviduct sampled 1
day after in vivo gene delivery
to oviducts. Oviducts were in vivo transfected with pCRTEIL alone (negative control),
pCRTEIL and pCAG/NCre (experiment), pCAG/NCre alone (negative control), or pCL
(positive control). Oviducts were isolated from each mouse at 2 days following
electroporation to measure luc activity. From a total of 8 females injected
with DNA, 4 oviducts were subjected to measurement of luc activity for each
transfection group. Note also that the vertical axis of the figure is
interrupted and has one scale. Different letters indicate statistically
significant differences ().
