Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector

Figure 4

Gene gun-mediated skin transfection with pCETRIZ (a)–(d) and pCETRIZ + pCAG/NCre (e)–(h). After shaving of the abdominal area of mice, plasmid DNA coated with gold particles was transfected with a 300-psi helium gas pulse using the Helios gene gun. Two days after gene delivery, skin was dissected and inspected for fluorescence under a UV illuminator. After observation for fluorescence, the same sample was then subjected to histochemical staining for lacZ activity. Figures (a)–(c) and (e)–(g) are photographs of the same portion. Figure (d) is a different portion from those in Figures (a)–(c). Similarly, Figure (h) is a different portion from those in Figures (e)–(g). (a), (d), (e), and (h) taken under light; (b) and (f): taken under UV illumination for detection of GFP fluorescence; (c) and (g): taken UV illumination for detection of red fluorescence. Bar: 10 𝜇m.