Impact of Lentiviral Vector-Mediated Transduction on the Tightness of a Polarized Model of Airway Epithelium and Effect of Cationic Polymer Polyethylenimine
Figure 1
Efficiency and cytotoxicity of LV transduction. Polarized 16HBE41o- cells were incubated with different MOIs for 24 (a, b, and c) or 4 (d, e, and f) hours and then analyzed either immediately for membrane permeability (b and e) and viability (c and f) or incubated for further 48 hours and evaluated for GFP expression (a and d). Percentage of propidium iodide (PI)-positive cells incubated with medium only was 3.45 0.28% and subtracted from the other values. In the viability assay, untreated cells were incubated with medium only and considered as 100%. Data are expressed as means SD of two-three experiments. A and B: for 500 and 2000 MOIs versus 50 and 100 MOIs. C and F: for 500 and 2000 MOIs versus mock.
