Expression of Nestin, Vimentin, and NCAM by Renal Interstitial Cells after Ischemic Tubular Injury

Table 1

Antibodies used for the immunohistochemical characterization of interstitial cells in rat kidney.

Primary antibodies	Origin	Dilution
AntiBrdU (Bromodeoxyuridine)	Mouse monoclonal (Bu20a)^∗—Dako, DK	1 : 20
AntiPCNA	Mouse monoclonal (PC10)^∗—Dako, DK	1 : 40
Antivimentin	Chicken, polyclonal—Chemicon, USA	1 : 1000
Antirodent nestin	Mouse monoclonal (Rat-401)^∗—Chemicon, USA	1 : 100
Anti-NCAM (Neural cell adhesion molecule)	Rabbit, polyclonal—Chemicon, USA	1 : 100
Antihuman 𝛼-SMA (Smooth muscle actin)	Rabbit, polyclonal—Abcam, UK	1 : 200

∗Antibody-producing clone.