The Expression of Soluble and Active Recombinant Haemophilus influenzae IgA1 Protease in E. coli
Figure 4
Expressed soluble IgA1 proteases were active. IgA1 was incubated with cell lysates or soluble supernatant at 37°C overnight. SDS-PAGE (reduced condition) and Western blotting with anti-IgA Ab were employed to detect the cleavage. IgA1 was cleaved by soluble supernatant (or cell lysate) induced at 12°C with 0.4 mM IPTG (except some were induced at 37°C with 1 mM IPTG for 3 hours).
