The Expression of Soluble and Active Recombinant Haemophilus influenzae IgA1 Protease in E. coli
Figure 5
Expression of soluble IgA1 protease from pET-IGAN in C41 (DE3) strain. SDS-PAGE (reduced condition) and Western blotting with anti-His Ab were employed to confirm the expression of IgA1 protease. Negative control: un-induced pET-IGAN C41 (DE3) soluble supernatant; purified IgA1 protease: purified IgA1 protease from H. influenzae.