Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection
Figure 3
Long-term effect of combined ionizing radiation (IR) and parvovirus H-1 (H-1PV) infection on human high-grade glioma cells. Short-term cultures of human glioblastoma NCH-89 were irradiated with 20 Gy, and infected with H-1PV at an MOI of 5 PFU/cell 24 hours post-IR. 3-week postseeding photographs of culture dishes were taken at a magnification of 400 and no surviving cells could be found (a). In comparison, after mono treatment with H-1PV at an MOI of 5 PFU/cell or after mono treatment with IR (data not shown), proliferating cell clones could be observed (b).
