The Collagen V Homotrimer [𝛼1(V)]_𝟑 Production Is Unexpectedly Favored over the Heterotrimer [𝛼1(V)]_𝟐𝛼2(V) in Recombinant Expression Systems

Figure 7

Double immunofluorescence staining was performed using monoclonal antibody against human pro𝛼1(V) chain (18G5) (a, d, g, j) and rabbit polyclonal pro𝛼2(V) chain (b, e, h, m), and merge (c, f, i, l). (a, b, c) human dermal fibroblasts; (d, e, f) clone 22 cells; (g)–(l) HeLa cells transiently transfected with human pro𝛼1(V) and pro𝛼2(V) constructs. Double immunofluorescence staining shows distinct intracellular distribution of the pro𝛼(V) chains in round (g)–(i) and spreading (j, k, l) HeLa cells. Magnification: x1000.