Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes
Figure 4
The pharmacological reactions of the P19CL6-A1-derived cardiomyocytes. The contraction rhythms of the beating cells are analyzed on the moving images before and after addition of the serial dilutions of IBMX (a) and ouabain (c) using the VisoRhythm software on which the temporal variations in the correlation coefficient are calculated between the values of mean brightness of the reference frame and those of other frames in the selected area ( pixels in white), and accordingly, the correlograms representing the contraction rhythm of the beating cells are displayed by the vertical coefficient and horizontal time axis. ((b) and (d)) The median effective doses (ED50) of IBMX (b) and ouabain (d) obtained from the dose-response curve based on the beating rates, on which the basal and maximum rates are represented by 0 and 100%, are 950 nM and 160 nM, respectively.
