Regression of Human Prostate Tumors and Metastases in Nude Mice following Treatment with the Recombinant Oncolytic Vaccinia Virus GLV-1h68

Figure 1

Cytotoxicity (a) and replication (b) of GLV-1h68 virus in human prostate DU-145 and PC-3 cells. (a) Viability of DU-145 and PC-3 cells after GLV-1h68 infection at MOIs of 0.1 and 1, respectively, monitored over 72 hours. Values are shown as percentages of respective uninfected controls. (b) Viral titer analysis in DU-145 and PC-3 cell cultures after infection with GLV-1h68 at an MOI of 0.1. Supernatants were collected from virally treated cells at various time points postinfection (hpi). Viral titers were determined as pfu per well in triplicate by plaque assay in CV-1 cell monolayers. Average plus standard deviation are plotted.