Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation

Table 1

Primer sequence used in pFastbac Dual vector construction.

Primer	Sequence 5^′-3^′
HA forward	GATCCGCCACC ATGAAATTCTTAGTCAACGTTGCCC
HA reverse	GCGGCCGCCCGTCTTCCATCTTCTTGTTTAAATTCT
NA forward	CTCGAGGCCACCATGAATCCAAACCAGAAAATAAT
NA reverse	GGTACCCTACTTGTCAATGGTGAACG
M1 forward	CTCGAGGCCACCATGAGTCTTCTAACCGAGGTC
M1 reverse	GGTACCTCACTTGAATCGTTGCATCTG
M2 forward	GAATCCGCCACCATGAGTCTTCTAACCGAGGT
M2 reverse	AAGCTTTTACTCCAGCTCTATGTTGA
NA-EGFP forward-1	CTCGAGGCCACCATGAATCCAAACCAGAAAATAATA
NA-EGFP forward-2	CATTACCTATAAAGTTGTTGCTGGGGTGAGCAAGGGCGAGGAG
NA-EGFP reverse-1	TGAACAGCTCCTCGCCCTTGCTCACCCCAGCAACAACTTTATAGGT
NA-EGFP reverse-2	GGTACCCTATTACTTGTACAGCTCGTC