Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV
Figure 4
(a) HPLC-chromatogram of pure mitoxantrone (MTO 200 ng/mL), (b) HPLC-chromatogram of processed blank liver tissue. No interfering peak at the retention time is determined for MTO. Chromatographic Conditions: mm X-Bridge Phenyl column (Waters, Germany), flow: 1 mL/min, mobile phase: buffer (80 nM sodium formate and formic acid, pH 3.0) and methanol (80 : 20 v/v). Column temperature 55°C, injection volume 50 L, UV-detection: 254 nm. (c) HPLC-chromatogram of processed liver tissue containing MTO bound to ferrofluids. MTO is at retention time 9,77.
