Research Article

The Availability of a Recombinant Anti-SNAP Antibody in VHH Format Amplifies the Application Flexibility of SNAP-Tagged Proteins

Figure 1

Application of anti-SNAP antibodies in western blot experiments. (a) Detection of 100 and 10 ng of blotted purified recombinant GST-SNAP protein by means of the VHH antibodies 3C4B and 3G1B. (b) Detection of overexpressed GST-SNAP in bacterial total lysates using the same VHH antibodies. The secondary antibody anti-llama immunoglobulins used for detection recognized nonspecifically a further band of 32 kDa.
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(a)
658954.fig.001b
(b)