The Availability of a Recombinant Anti-SNAP Antibody in VHH Format Amplifies the Application Flexibility of SNAP-Tagged Proteins
Figure 2
Application of anti-SNAP antibodies in immunopurification experiments. (a) Purified GST-SNAP (0.5 or 0.1 g) were incubated in the presence of the biotinylated anti-SNAP VHH 3G1B (5 or 1 g), and the antigen-antibody complex was precipitated using avidin beads. The proteins were separated by SDS-PAGE, blotted, and finally detected using either anti-GST (upper membrane strip) or anti-VHH specific antibodies (lower membrane strip). Biotinylated VHHs were incubated in the presence of avidin beads and in the absence of GST-SNAP as a control of the pull-down efficiency. (b) Lysates from mammalian HEK293T cells transiently transfected with 20 g/plate plasmid for expressing the SNAP-Twist2 fusion construct were incubated with the anti-SNAP 3G1B-Fc(R) antibody, and the antigen-antibody complex was precipitated by means of Protein A.
