Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein

Figure 2

Structural organization of the E. histolytica DNA polymerases of family B. A graphical alignment of EhOPD1, EhODP2, EhODP3 and EhODP4 with DNA polymerases of different fungi mitochondrial plasmids and a putative DNA polymerase of T. vaginalis was obtained based on an alignment with the ClustalW program as described in Materials and Methods. Boxes I, II and III, marked in the bars, are specific to family B. Boxes A and B correspond to two conserved regions found in fungi organellar DNA polymerases and that from T. vaginalis. Numbers at the beginning of bars correspond to the first methionine, and at the end of the bars to the full length for each polypeptide. Arrows indicate the organellar DNA polymerases of E. histolytica. Nin: Neurospora intermedia; Gsp: Gelasinospora sp; Aim: Ascobolus immersus; Fve: Flammulina velutipes; Cpe: Crinipellis perniciosa; Aae: Agrocybe aegerita; Kla-2: Kluyveromyces lactis DNA polymerase 2, found in plasmid pGKL-2; Skl: Saccharomyces kluyveri; Pet: Pichia etchellsii; Mco: Morchella conica; Ppu: Porphyra purpurea; Pan: Podospora anserina; Kla-1: Kluyveromyces lactis DNA polymerase 1, found in plasmid pGKL-1; Pkl: Pichia kluyveri; Ehi-1: Entamoeba histolytica organellar DNA polymerase 1; Ehi-2: Entamoeba histolytica organellar DNA polymerase 2; Ehi-3: Entamoeba histolytica organellar DNA polymerase 3; Ehi-4: Entamoeba histolytica organellar DNA polymerase 4; Tva: Trichomonas vaginalis.