Optimization of Formaldehyde Cross-Linking for Protein Interaction Analysis of Non-Tagged Integrin 𝛽1

Table 2

Proteins identified in the integrin 𝛽1 complex precipitated from Jurkat cells. Formaldehyde cross-linked Jurkat cell lysate was used to enrich integrin 𝛽1 with JB1B. The integrin 𝛽1 complex was identified by SDS PAGE analysis and studied by LC-MS/MS and the GPM. Only proteins with a log(e) <−1 and which were identified in both datasets and not in control samples are listed.

Protein		Accession	Mr [kDa]	log(e) (Dataset 1)	log(e) (Dataset 2)
Integrin 𝛽1	ITGB1	ENSP00000303351	88.4	−39.8	−118.3
Actin, cytoplasmic 2	ACTG1	ENSP00000331514	41.8	−23.4	−73.1
Desmoglein-1	DSG1	ENSP00000257192	113.7	−16.4	−42.9
Filaggrin-2	FLG2	ENSP00000373370	247.9	−15.6	−40.1
Glyceraldehyde-3-phosphate dehydrogenase	GAPDHL6	ENSP00000229239	36	−7.4	−18.2
Arginase-1	ARG1	ENSP00000349446	35.6	−7.3	−15.1
Junction plakoglobin	JUP	ENSP00000377507	81.7	−6	−7
Integrin 𝛼4	ITGA4	ENSP00000380227	114.8	−5.1	−6.3
Heat shock protein HSP 90𝛼	HSP90AA2	ENSP00000216281	84.6	−2.9	−4.5
C1orf68	C1orf68	ENSP00000354769	26.2	−2.1	−3.2
Integrin 𝛼6	ITGA6	ENSP00000264106	126.5	−1.6	−2.4