Rapid Decrease of CD16 (FcγRIII) Expression on Heat-Shocked Neutrophils and Their Recognition by Macrophages
Figure 2
Recognition of heat-shocked neutrophils is nonphlogistic. (a) hMDMs were cocultured for 4 or 24 hrs with fresh, heat-shocked (39 or 41°C, 90 min), or necrotic neutrophils or stimulated with 10 ng/mL LPS (E. coli). TNF-α was subsequently measured in cell-free culture supernatants. Data presented are mean (± SD). ***, relative to control, PMN fresh. (b) hMDMs were cocultured for 1 hr with apoptotic, heat-shocked (39 or 41°C, 90 min), or necrotic neutrophils. The cocultures were then stimulated for 4 hrs with 10 ng/ml or 1 μg/ml LPS (E. coli). TNF-α was subsequently measured in cell-free culture supernatants. Results are expressed as percent (mean ± SD) of positive control that is hMDMs stimulated with LPS. *,**, relative to positive control. PMNs incubated in standard culture conditions up to 120 min had the same effect as freshly isolated cells (data not shown).
