Rapid Decrease of CD16 (FcγRIII) Expression on Heat-Shocked Neutrophils and Their Recognition by Macrophages
Figure 3
Expression of CD31 is modulated on heat-shocked neutrophils. Fresh, apoptotic, and HS neutrophils (incubated at 41°C for indicated time) were stained with PE-conjugated antihuman CD31 mAb and analyzed by flow cytometry. (a) Open bars represent the percent of CD31-positive cells; black bars represent mean fluorescent intensity (MFI) expressed as percent of MFI measured for control, fresh PMNs. (b) Flow cytometry histograms from the same experiment are presented. The histogram markers were set based on the signal from isotype control and they designated section of the histogram representing neutrophils positive for CD31. Results of a representative experiment of three performed are presented. PMNs incubated in standard culture conditions up to 120 min expressed CD31 with the same intensity as freshly isolated cells (Positive cells, 98.17 ± 3.09%; MFI, 6839 ± 640.88).
