Rapid Decrease of CD16 (FcγRIII) Expression on Heat-Shocked Neutrophils and Their Recognition by Macrophages

Figure 4

Surface expression of Fcγ receptor III (CD16) is reduced by heat shock. Neutrophils incubated for 90 min, at indicated temperatures were stained with PE-conjugated antihuman CD16 mAb and analyzed by flow cytometry. The histogram markers were set based on the signal from isotype control (Mouse IgG1-PE clone MOPC-21), and they designated section of the histogram representing neutrophils positive for CD16. The values of MFI are given in histograms. Results of a representative experiment of four performed are presented (complete data in Table 1).