Rapid Decrease of CD16 (FcγRIII) Expression on Heat-Shocked Neutrophils and Their Recognition by Macrophages

Figure 6

Geldanamycin and radicicol enhance the effect of HS on surface expression of CD16. Neutrophils were incubated initially for 30 min at 37°C with or without inhibitors of HSP90: geldanamycin (Ge) or radicicol (Rad) and then for additional 90 min at 37°C (control) or 41°C (heat shock). Finally, cells were stained with PE-conjugated antihuman CD16 mAb and analyzed by flow cytometry. The histogram markers were set based on the signal from isotype control (Mouse IgG1-PE clone MOPC-21) and they designated section of the histogram representing neutrophils positive for CD16. Results of a representative experiment of three performed are presented.