Development of Novel In Silico Model to Predict Corneal Permeability for Congeneric Drugs: A QSPR Approach

Table 2

Gradient mobile phase used during the analysis by HPLC-PDA for fluoroquinolones in group A and group B.

Time (min)	Methanol (%)	Acetonitrile (%)	Buffer (20 mM) (%)	Flow rate (mL/min)
Group A
0.01	0	10	90	1.0
4.00	5	15	85	1.0
10.0	0	40	60	1.0
12.0	0	10	90	1.0
Group B
0.01	10	10	80	1.0
6.00	10	10	80	1.0
8.00	0	50	50	1.0
10.0	10	10	80	1.0