Chromosomal Rearrangements in Post-Chernobyl Papillary Thyroid Carcinomas: Evaluation by Spectral Karyotyping and Automated Interphase FISH

Table 1

Karyotypes and RET/PTC rearrangements in papillary thyroid carcinomas from Ukraine.

			RET/PTC rearrangement (rearranged cells %)
Case	Age/gender	SKY analysis Clonal aberrations^#	No. of cells scored	Automated scoring with Metafer4	Visual scoring of Metafer4 recorded cells
RPE (control w/o RET/PTC)	—/f	—	186	0.7	1.1
TPC1 (control with RET/PTC)	—/f	der(1)t(1;3), der(1)t(1;21), del(3p), i(8p), der(10)t(10q;3p), der(10)t(10q;1q); del(10p), −21	103	98.1	97.1
S414N (control w/o RET/PTC)	33/f	n.a.	180	3.1	1.1
S399T	13/m	—	103	17.7^+	8.7^+
S400T	19/m	−7, −12, −21, −20	354	13.3^+	16.1^+
S402T	34/m	−19, −11, i(11)(q10)	139	12.1^+	10.8^+
S403T	12/m	del(11p)	n.a.
S404T	16/f	−11, −20, −21, −22	154	11.6^+	11.7^+
S405T	16/f	−10, −12	233	19.4^+	21.0^+
S407T	19/f	−10, −16, −18, −20, −21	608	5.4*	10.7^*+
S408T	25/m	−2, −5, −7, −10,−13, −17, −18, −22	715	2.1	1.5
S409T	28/f	n.a.	122	10.6^+	13.9^+
S411T	12/f	—	793	2.2	2.0
S412T	21/f	−7, −8, −21	267	12.9^+	9.0^+
S413T	18/f	−14, −21	220	5.2	5.9^+
S414T	33/f	−20	67	23.8^+	20.9^+
S416T	15/m	—	211	1.0	2.4
S418T	27/m	−16	262	12.9^*+	3.1*
S420T	28/f	—	190	14.2^+	9.5^+
S422T	31/m	—	756	23.2^*+	14.6^*+
S428T	21/m	—	683	11.0^+	16.8^+
S429T	13/f	−9, −22	330	20.6^+	16.7^+
S430T	32/f	—	102	41.5^+	33.3^+
S431T	21/f	−9, −19, −20	110	33.8^+	30.0^+
S432T	26/m	n.a.	80	12.2^+	12.5^+
S437T	22/f	−21	126	15.1^+	13.5^+

^#At least 15 metaphases were analysed by SKY.
—: normal karyotype.
n.a.: not analysed.
*Significant difference between automated and visual scoring (χ ² test, 𝑃<.05; Bonferroni value).
⁺ Significant difference to respective negative control (S414N; Fisher's exact test, 𝑃<.05).