Chromosomal Rearrangements in Post-Chernobyl Papillary Thyroid Carcinomas: Evaluation by Spectral Karyotyping and Automated Interphase FISH

Table 2

Classifier parameters for cell and signal selection.

Parameters	Variability of parameters	Finally optimized parameters
Cell selection parameters
Minimum cell nucleus area	0 to 32,000 μm^2	120 μm^2
Maximum cell nucleus area	0 to 32,000 μm^2	500 μm^2
Maximum concavity depth	0 to 1	0.35
Maximum aspect ratio	1 to 10	2.5
Signal parameters
Min. absolute signal area	0 to 100 μm^2	0.1 μm^2
Max. distance of fused signals	0.1 to 100 μm	0.8 μm
Min. of max. signal intensity	0 to 100%	33%