BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology

Table 2

Variant specificity.

LoxP spacer pairs	Inversion	Deletion	wt
	171 bp	240 bp	360 bp
LoxP-LoxP	51.7%	48.3%	0.0%
Loxm2-Loxm2	0.0%	99.3%	0.7%
LoxP-Loxm2	49.0%	0.7%	50.3%
Loxm2-Lox2722	0.0%	0.0%	100.0%
Loxm2-Lox5171	0.0%	0.0%	100.0%
Lox2722-LoxP	0.0%	0.0%	100.0%
LoxP-Lox5171	55.2%	0.0%	44.8%
Lox2722-Lox5171	0.0%	0.0%	100.0%

The percentage of clones of each type product (inversion, deletion, or WT) present in a PCR band is indicated Lox spacer pairs. Inversion indicates a reaction involving the LoxP site in the pBelo BAC backbone (inverted spacer repeat) while deletions indicate a reaction between the indicated Lox pair (direct spacer repeats). Percentages are based on positives out of 144 samples.