BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology

Table 3

Primers used for generating recombineering targeting fragments (Sections 3.4 and 3.6).

MEP-086	B1_5^'_61727-61776	CCA  GCG  CCT  ACA  GCG  CCC  CAA  CCT  CTT  TTC  CCC  CCT  GCT  CAG  CTCCGG  CCA  TGC  CAG  AGC  CAG  CGA  AGT  CTG  C
MEP-087	B1_3^'_61797-61846	CCG  AGT  TTT  GTT  GGA  GCG  CTG  AGC  TAG  GCA  GCC  CTC  CAC  CAT  AGCGGC  TCA  ATT  CGC  CCT  TCC  CGG  GCG
MEP-088	B2_5^'_49041-49090	GCG  GGC  CGA  GCG  CCC  AGG  ACG  ACG  CGG  GAG  AGC  CAG  CCG  AGG  AACCCA  CGA  TGC  CAG  AGC  CAG  CGA  AGT  CTG  C
MEP-089	B2_3^'_49111-49160	CCT  GCG  CGG  CCT  CGG  CGG  GGT  GGA  AGC  AGG  CTT  CCC  GCA  GCC  GGTGAG  TCA  ATT  CGC  CCT  TCC  CGG  GCG
MEP-090	B3_5^'_40146-40195	ACT  CCT  CGT  ACC  CTG  GCA  GCA  ATG  GTT  TCG  GCT  ACG  ACG  GGC  CTCCCC  AGA  TGC  CAG  AGC  CAG  CGA  AGT  CTG  C
MEP-091	B3_3^'_40216-40265	CCA  GGG  ACT  GCA  GAG  AAC  ACG  CTG  AGC  GCT  GGT  AGT  CAC  CCT  CCAGGT  GCA  ATT  CGC  CCT  TCC  CGG  GCG
MEP-092	B4_5^'_14665-14714	CTC  CGT  GCG  AGG  AGT  ATT  CAC  AGA  GCG  ATT  ACC  TAC  CCA  GCG  ACCACT  CGA  TGC  CAG  AGC  CAG  CGA  AGT  CTG  C
MEP-093	B4_3^'_14735-14784	CCC  GGC  GCC  CAA  AGG  CCG  CCT  CCG  GCT  GGA  AGC  CGC  TCT  CTC  GCCTCT  GGA  ATT  CGC  CCT  TCC  CGG  GCG