Research Article
Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad
Table 2
Primers used for real-time RT-PCR analysis.
| | Genes | Primer name | Primer sequences 5′→3′ |
| | Sry | Sry-F | AGCTCTTACACTTTAAGTTTTGAC | | Sry-R | GCAGCTCTACTCCAGTCTTGCC | | Wt1(−KTS) | Wt1(−KTS)-F | TACCTTAAAGGGCCACGGTAT | | Wt1(−KTS)-S | GAAGGGCTTTTCACCTGTATGA | | Wt1(+KTS) | Wt1(+KTS)-F | GCTGGGAGCTCCAGCTCAGT | | Wt1(+KTS)-R | GGGCTTTTCACTTGTTTTAC | | Sox9 | Sox9-F | GGTGCTGAAGGGCTACGCTGGACG | | Sox9-R | GGCTGGTACTTGTAATCGGGTGGTC | | Gata4 | Gata4-F | CGAGGGTGAGCCTGTATGTAA | | Gata4-R | TGGCCTGCGATGTCTGAGTG | | Amh | Amh-F | TCCTACATCTGGCTGAAGTGATATGGGAGC | | Amh-R | CTCAGGGTGGCACCTTCTCTGCTTGGTTGA | | Sf1 | Sf1-F | TGGTGTCCAGTGTCCACCCTTAT | | Sf1-R | TCCGTCACGTGTAATGCTTGT | | Dax1 | Dax1-F | AGAGTATGCCTATCTGAAAGGGACCGTG | | Dax1-R | ACGACATCGCTATTGATGAATCTCAGCA | | β-actin | β-actin-F | AACCCTAAGGCCAACCGTG | | β-actin-R | CAGGATTCCATACCCAAGAAGG |
|
|
