Research Article
A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus
Table 3
Comparison of biochemical characteristics and activities of laccase from Lentinus tigrinus and other mushroom laccases.
| | Lentinus tigrinus HPXG59 | Panus tigrinus CBS 577.79 | Panus tigrinus 8/18 | Panus tigrinus BKM F3616D | Tricholoma mongolicum | Lentinus edodes | Lentinus edodes (lacI) | Lentinus edodes (lacII) | Pleurotus eryngii | Hericium erinaceus | Tricholoma giganteum |
| | Molecular mass (kDa) | 59 | 69.1 | 63 | — | 66 | 67 | 72 | 58 | 34 | 63 | 43 | | Chromatographic behavior on | | | | | | | | | | | | | DEAE-ion exchange | AD | — | AD | AD | AD | UN | AD | — | AD | AD | UN | | CM-ion exchange | AD | — | — | — | AD | UN | — | AD | UN | UN | AD | | Q-ion exchange | — | AD | AD | — | UN | — | AD | — | AD | AD | — | | Affi-Gel blue gel | — | — | — | — | — | UN | — | — | — | — | AD | | Optimum pH | 4.0 | 3.75 | 4.8 | 7.0 | 2-3 | 4.0 | 4 | 3 | — | 5 | 4 | | Optimum temperature (°C) | 60 | 55 | 60 | 65 | 30 | 70 | 40 | 40 | 70 | 50 | 70 | | HIV-1 reverse transcriptase inhibitory activity (IC50) | 2.4 μM | — | — | — | 0.65 μM | 7.5 μM | — | — | 2.2 μM | 9.5 μM | 2.2 μM |
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AD: adsorbed. UN: Unadsorbed. —: not determined or not attempted.
References: Panus tigrinus CBS 577.79 [29], Panus tigrinus BKM F3616D [30], Panus tigrinus 8/18 [31–34], Tricholoma mongolicum [35], Lentinus edodes [36], Lentinus edodes (lacI) [37], Lentinus edodes (lacII) [38], Pleurotus eryngii [39], Hericium erinaceus [19], Tricholoma giganteum [18].
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