Progesterone Downregulates Oestrogen-Induced Expression of CFTR and SLC26A6 Proteins and mRNA in Rats’ Uteri

Figure 2

Real-time PCR quantitation (a), and Western blot analysis (b), and Western blot photo (c) of SLC26A6 in ovariectomized rats and rats at different stages of the oestrous cycle. SLC26A6 is from Cl⁻/HCO₃⁻ exchanger family that is upregulated by oestrogen and at Ps and Es stages of the oestrous cycle. Control: peanut oil., E: 0.2 μg oestrogen, 20 E: 20 μg oestrogen, 50 E: 50 μg oestrogen, P: progesterone, E + vehicle: 0.2 μg oestrogen + peanut oil E + P: 0.2 μg oestrogen + progesterone. Ps: proestrus, Es: estrus, Ms: metestrus, Ds: diestrus: *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001.

(a)

(b)

(c)