Persistent Dystrophin Protein Restoration 90 Days after a Course of Intraperitoneally Administered Naked 2′OMePS AON and ZM2 NP-AON Complexes in mdx Mice
Figure 5
Western blot analysis. Immunoblotting for dystrophin using DYS2 antibody showed restored expression of the protein in the quadriceps and diaphragm of ZM2–AON-treated mice (lane 4), while no protein was detected in either untreated control (NT, lane 2) or naked AON-treated mdx mice (lane 3). Dystrophin protein was undetectable in the hearts of both untreated and treated mdx mice. Quadriceps, diaphragms, and hearts from WT mice were used as positive controls (lane 1). For WT samples, the total protein loaded was 1/10 (15 μg) of the quantity in the other lanes (150 μg). Quantification performed by densitometric analysis of autoradiographic bands followed by normalization with the quantity of total protein loaded onto the gel showed a dystrophin recovery of 8 ± 0.6% in quadriceps and 7 ± 0.17% in diaphragm () of ZM2–AON-treated mice with respect to WT control mice. The results are representative of three separate experiments. Data are given as means ± S.E.M. and the statistical significance has been calculated with the Student’s t-test for unpaired data.
