Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples

Table 1

Primers and probes used in this study.

Target	Purpose	Primer sequence (5′→3′)	Amplicon size
Kefeng6	Construction	KF6-1F  GGATCCACGTAGTACGTACCGCCGTG^1	89
		KF6-1R  GGATCCAGTGCAGATGCATGAATCGC^1
	Quantification	KF6-2F  ACGTAGTACGTACCGCCGTG	77
		KF6-2R  AGTGCAGATGCATGAATCGC
		KF6-P  FAM-CCGCGCGTTGTACTGAGAACCA-TAMRA
gos9	Construction	gos9-1F  GGATCCTTAGCCTCCCGCTGCAGA^1	80
		gos9-1R  AAGCTTAGAGTCCACAAGTGCTCCCG^2
	Quantification	gos9-2F  TTAGCCTCCCGCTGCAGA	68
		gos9-2R  AGAGTCCACAAGTGCTCCCG
		gos9-P  FAM-CGGCAGTGTGGTTGGTTTCTTCGG-TAMRA
Kefeng6 and gos9	Fusion	Fusion-F  GGGAGGCTAAGGATCCAGTGCAGATG	183
		Fusion-R  CATCTGCACTGGATCCTTAGCCTCCC

1BamHI site in bold type, ²HindIII site in bold type.