Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem

Table 2

Effect of protease inhibitors, metal ions, and solvents on proteolytic activity of B. amyloliquefaciens MA20.

Substance	Final concentration	Relative activity (%)	Final concentration	Relative activity (%)
Control	0	100	0	100
β-mercaptoethanol	5 mM	18	1 mM	98
PMSF	5 mM	0	1 mM	0
EDTA	5 mM	35	1 mM	97
SDS	0.5%	54	0.1%	122
ZnCl2	10 mM	153	5 mM	156
MgCl2	10 mM	119	5 mM	95
CuSO4	10 mM	266	5 mM	204
Urea	10 mM	114	5 mM	139
HgCl2	10 mM	100.4	5 mM	107
CaCl2	10 mM	104	5 mM	107
BaCl2	10 mM	115	5 mM	116
Guanidin HCl	10 mM	103	5 mM	132
MnCl2	10 mM	71	5 mM	162
Methanol	1%	90	0.5%	100
Ethanol	1%	103	0.5%	100
DMSO	1%	90	0.5%	103
Isopropanol	1%	98	0.5%	108
Tween 20	1%	86	0.5%	94
Triton X100	1%	61	0.5%	65